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| Status |
Public on Dec 08, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Il17ra villin cre -/+ colon transcriptomes at day 10 post C. rodentium infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report analysis of colonic transcriptome in gut epithelial cells specific deficient mouse (Il17ra villin cre +) and littermate control mouse (Il17ra villin cre -) infected with Citrobater rodentium (C. rodentium) for 10 days. We found RNA-seq data show Nox family genes and defensin genes are downregulated in Il17ra villin cre + mice. It is interestingly Tnfsf13 was also downregulated in Il17ra villin cre + mice. These genes expressions were confirmed by qRT–PCR validation was performed using TaqMan assays. Finally, this study provides first detailed analysis of C. rodentium infected colonic transctiptome in Il17ra villin cre + mice, with biologic replicates, generated by RNA-seq technology.
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| Overall design |
colonic mRNA profiles of Il17ra villin cre -/+ at day 10 post C. rodentium infection
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| Contributor(s) |
Kolls JK, Matsunaga Y |
| Citation(s) |
33431657 |
| Submission date |
Oct 26, 2020 |
| Last update date |
Mar 09, 2021 |
| Contact name |
Jay Kolls |
| Organization name |
Tulane University
|
| Department |
Medicine
|
| Lab |
Center for Translational Research in Infection and Inflammation
|
| Street address |
1430 Tulane Ave
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA672119 |
| SRA |
SRP288639 |
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