 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 01, 2020 |
| Title |
Characterization of Mutational Status, Spheroid Formation, and Drug Response of a New Genomically-Stable Human Ovarian Clear Cell Carcinoma Cell Line, 105C (Affymetrix) |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
SNP genotyping by SNP array
|
| Summary |
Ovarian clear cell carcinoma (OCCC) is a rare subtype of gynecological cancer for which well-characterized and authenticated model systems are scarce. We provide an extensive characterization of ‘105C’, a cell line generated from an adenocarcinoma of the clear cell histotype using targeted next-generation sequencing, cytogenetic microarrays, along with analyses of AKT/mTOR signaling. We report that that the 105C cell line is a bona fide OCCC cell line, carrying PIK3CA, PTEN, and ARID1A gene mutations, consistent with OCCC, yet maintain a stable genome as reflected by low copy number variation. Unlike KOC-7c, TOV-21G, and RMG-V OCCC lines also mutated for the above genes, the 105C cells do not carry mutations in mismatch repair genes. Importantly, we show that 105C cells exhibit greater resistance to mTOR inhibition and carboplatin treatment compared to 9 other OCCC cell lines in 3D spheroid cultures. This resistance may be attributed to 105C cells remaining dormant in suspension culture which surprisingly, contrasts with several other OCCC lines which continue to proliferate in long-term suspension culture. 105C cells survive xenotransplantation but do not proliferate and metastasize. Collectively, we show that the 105C OCCC cell line exhibits unique properties useful for the pre-clinical investigation of OCCC pathobiology.
Copy number variation was determined for multiple Ovarian Clear Cell Carcinomas
|
| |
|
| Overall design |
DNA from various OCCCs was analyzed using cytoscanHD and copy number was determined using Chromosome Analysis Suite (Applied Biosystems/Thermo Fisher Scientific, version 4.0.0.385)
|
| |
|
| Contributor(s) |
Kolendowski B, Ramos-Valdes Y, Hirte H, Itamochi H, Lee W, Carey M, Shepherd TG, DiMattia GE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 31, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Bart Kolendowski |
| Organization name |
Lawson Research
|
| Street address |
750 Base Line Rd E
|
| City |
London |
| State/province |
ONTARIO |
| ZIP/Postal code |
N6C2R5 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16131 |
[CytoScanHD_Array] Affymetrix CytoScan HD Array |
|
| Samples (6)
|
| GSM4875507 |
Ovarian Clear Cell Carcinoma cell line 105C |
| GSM4875508 |
Ovarian Clear Cell Carcinoma cell line KOC-7c |
| GSM4875509 |
Ovarian Clear Cell Carcinoma cell line SMOV-2 |
| GSM4875510 |
Ovarian Clear Cell Carcinoma cell line OVSAYO |
| GSM4875511 |
Ovarian Clear Cell Carcinoma cell line TU-OC-1 |
| GSM4875513 |
Ovarian Clear Cell Carcinoma cell line 105CT |
|
| This SubSeries is part of SuperSeries: |
| GSE160571 |
Characterization of Mutational Status, Spheroid Formation, and Drug Response of a New Genomically-Stable Human Ovarian Clear Cell Carcinoma Cell Line, 105C |
|
| Relations |
| BioProject |
PRJNA673622 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160569_RAW.tar |
659.2 Mb |
(http)(custom) |
TAR (of CEL, CYCHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...