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| Status |
Public on Sep 08, 2009 |
| Title |
The non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Vault particles are conserved organelles that have been implicated in multidrug resistance and intracellular transport. They are formed by three different proteins and the non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from the canonical microRNA (miRNA) pathway. At least one of these svRNAs, we named svRNAb, associates with Argonaute proteins to guide sequence-specific cleavage and regulate gene expression in a manner similar to miRNAs. We demonstrate that svRNAb downregulates CYP3A4, a key enzyme in drug metabolism. Our findings expand the repertoire of small regulatory RNAs and assign, for the first time, a function to vRNAs that may help explain the observed association between vaults and drug resistance.
Keywords: Transcriptome analysis
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| Overall design |
Small RNAs 18-35 nt were isolated from MCF7 cell total RNA and sequenced on the Illumina Genome Analyzer
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| Contributor(s) |
Persson H, Kvist A, Vallon-Christersson J, Medstrand P, Borg A, Rovira C |
| Citation(s) |
19749744 |
| Submission date |
May 12, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Helena Persson |
| E-mail(s) |
Helena.Persson@med.lu.se
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| Organization name |
Lund University
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| Department |
Dept of Clinical Sciences, Oncology
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| Street address |
Scheelevagen 2
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| City |
Lund |
| ZIP/Postal code |
SE-22381 |
| Country |
Sweden |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (1) |
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| Relations |
| SRA |
SRP001310 |
| BioProject |
PRJNA115477 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data included within Sample table |
| Raw data are available in SRA |
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