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Series GSE16062 Query DataSets for GSE16062
Status Public on May 20, 2009
Title Generation of Induced Pluripotent Stem Cells using Recombinant Proteins
Organism Mus musculus
Experiment type Expression profiling by array
Summary Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human iPSCs were subsequently generated using similar genetic manipulation (Takahashi et al., 2007,Yu et al., 2007). To address the safety issues arose from harboring integrated exogenous sequences in the target cell genome, a number of modified genetic methods have been developed and produced iPSCs with potentially reduced risks (for discussion, see Yamanaka, 2009, and references therein). However, all of the methods developed to date still involve the use of genetic materials and thus the potential for unexpected genetic modifications by the exogenous sequences in the target cells. Here we report generation of protein-induced pluripotent stem cells (piPSCs) from murine embryonic fibroblasts using recombinant cell-penetrating reprogramming proteins. We demonstrated that such piPSCs can long-term self-renew and are pluripotent in vitro and in vivo.
 
Overall design Global gene-expression analyses of the piPS cells
 
Contributor(s) Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, Trauger S, Bien G, Yao S, Zhu Y, Siuzdak G, Schöler HR, Duan L, Ding S
Citation(s) 19398399
Submission date May 12, 2009
Last update date Jun 14, 2018
Contact name Hongyan Zhou
Organization name the scripps research institute
Street address 10550 North Torrey Pines Road
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM402330 piPSC, biological replicate 1
GSM402331 piPSC, biological replicate 2
GSM402332 OG2-MEF, biological replicate 1
Relations
BioProject PRJNA115481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16062_RAW.tar 3.1 Mb (http)(custom) TAR
GSE16062_non-normalized.txt.gz 1.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record
Raw data provided as supplementary file

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