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| Status |
Public on May 20, 2009 |
| Title |
Generation of Induced Pluripotent Stem Cells using Recombinant Proteins |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human iPSCs were subsequently generated using similar genetic manipulation (Takahashi et al., 2007,Yu et al., 2007). To address the safety issues arose from harboring integrated exogenous sequences in the target cell genome, a number of modified genetic methods have been developed and produced iPSCs with potentially reduced risks (for discussion, see Yamanaka, 2009, and references therein). However, all of the methods developed to date still involve the use of genetic materials and thus the potential for unexpected genetic modifications by the exogenous sequences in the target cells. Here we report generation of protein-induced pluripotent stem cells (piPSCs) from murine embryonic fibroblasts using recombinant cell-penetrating reprogramming proteins. We demonstrated that such piPSCs can long-term self-renew and are pluripotent in vitro and in vivo.
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| Overall design |
Global gene-expression analyses of the piPS cells
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| Contributor(s) |
Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, Trauger S, Bien G, Yao S, Zhu Y, Siuzdak G, Schöler HR, Duan L, Ding S |
| Citation(s) |
19398399 |
| Submission date |
May 12, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Hongyan Zhou |
| Organization name |
the scripps research institute
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| Street address |
10550 North Torrey Pines Road
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA115481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16062_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE16062_non-normalized.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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