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| Status |
Public on Jan 25, 2021 |
| Title |
SLAMF5 blocking of sorted Bregs CD19+IL10+ (GFP+) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Our results show that in the absence of SLAMF5 ( by KO or blocking ab), there is an accumulation of Bregs (CD19+IL10+) and a specific increase of their survival. Here, using RNAseq, we wished to identify genes that take place in the regulation of Breg by SLAMF5 blocking.
Method: Total RNA was extracted from 3x10e5 sorted CD19+GFP+cells from 2-3 Vert-x mice (induced with EAE) incubated with SLAMF5 blocking Ab or control IgG using the Direct-zol RNA kit (ZymoResearch) according to the manufacturer’s instructions. RNA-seq was performed using the MARS-seq protocol. Showing here 2 biological replicates of each group.
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| Overall design |
RNA of CD19+IL10+ sorted cells (il10 GFP reporter mice) derived of EAE sick mice, incubated with SLAMF5 blocking Ab or control IgG.
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| Contributor(s) |
Radomir L, Shachar I |
| Citation(s) |
33767202 |
| Submission date |
Nov 04, 2020 |
| Last update date |
Apr 20, 2021 |
| Contact name |
Idit Shachar |
| E-mail(s) |
idit.shachar@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
immunology
|
| Street address |
Herzl 234
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA674605 |
| SRA |
SRP291130 |
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