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Series GSE161253 Query DataSets for GSE161253
Status Public on Nov 12, 2020
Title Effects of polycyclic aromatic hydrocarbons on osteoclasts and osteoblasts in the scales of zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary It has been reported that polycyclic aromatic hydrocarbons (PAHs) act on calcified tissue and suppress osteoblastic activity in the scales of teleost fish.
In the present study, the differentially-expressed genes in the zebrafish scales treated with benzo[c]phenanthrene (BcP), a kind of PAH, or its metabolite 3-hydroxybenzo[c]phenanthrene (3-OHBcP) were investigated using GeneChip® oligonucleotide microarrays.
 
Overall design Zebrafish scales were used. The scales were incubated at 15 °C for 6 h in Leibovitz's L-15 medium (Invitrogen, Grand Island, NY, USA) with benzo[c]phenanthrene (BcP: 1 nM) or 3-hydroxybenzo[c]phenanthrene (3-OHBcP: 1 nM). Total RNA samples were prepared from the scales, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was analyzed by an Affymetrix GeneChip® system with a GeneChip Zebrafish Genome array. Sample preparation for array hybridization was carried out as described in the manufacturer’s instructions.
 
Contributor(s) Tabuchi Y, Suzuki N
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Submission date Nov 11, 2020
Last update date Nov 14, 2020
Contact name Yoshiaki Tabuchi
E-mail(s) ytabu@cts.u-toyama.ac.jp
Organization name University of Toyama
Department Life Science Research Center
Lab Division of Molecular Genetics Research
Street address 2630 Sugitani
City Toyama
State/province Toyama
ZIP/Postal code 930-0194
Country Japan
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (3)
GSM4904002 1_Ctr: Control
GSM4904003 2_BcP: benzo[c]phenanthrene (BcP)-treated
GSM4904004 3_3-OHBcP: 3-hydroxybenzo[c]phenanthrene (3-OHBcP)-treated
Relations
BioProject PRJNA676042

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161253_RAW.tar 5.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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