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| Status |
Public on Nov 12, 2020 |
| Title |
Effects of polycyclic aromatic hydrocarbons on osteoclasts and osteoblasts in the scales of zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
It has been reported that polycyclic aromatic hydrocarbons (PAHs) act on calcified tissue and suppress osteoblastic activity in the scales of teleost fish.
In the present study, the differentially-expressed genes in the zebrafish scales treated with benzo[c]phenanthrene (BcP), a kind of PAH, or its metabolite 3-hydroxybenzo[c]phenanthrene (3-OHBcP) were investigated using GeneChip® oligonucleotide microarrays.
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| Overall design |
Zebrafish scales were used. The scales were incubated at 15 °C for 6 h in Leibovitz's L-15 medium (Invitrogen, Grand Island, NY, USA) with benzo[c]phenanthrene (BcP: 1 nM) or 3-hydroxybenzo[c]phenanthrene (3-OHBcP: 1 nM). Total RNA samples were prepared from the scales, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was analyzed by an Affymetrix GeneChip® system with a GeneChip Zebrafish Genome array. Sample preparation for array hybridization was carried out as described in the manufacturer’s instructions.
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| Contributor(s) |
Tabuchi Y, Suzuki N |
| Citation missing |
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| Submission date |
Nov 11, 2020 |
| Last update date |
Nov 14, 2020 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
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| Organization name |
University of Toyama
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| Department |
Life Science Research Center
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| Lab |
Division of Molecular Genetics Research
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| Street address |
2630 Sugitani
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| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA676042 |
