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| Status |
Public on Nov 17, 2020 |
| Title |
Super-enhancer landscape shaped by master regulator in liver metastasis of colorectal cancer [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We interrogated global super-enhancer landscape and sought to discover master regulators governing the liver metastasis of colorectal cancer (CRC). Using de novo analysis, we predicted motif enrichment of master transcription factor in super-enhancers. We show that transcription factor-binding sites of hepatocyte nuclear factor 1 (HNF1) family are enriched at super-enhancers identified in cell lines derived from liver metastases, but not those derived from primary tumors. Using ChIP-Seq of acetylated H3K27, the enrichment of HNF1 binding motifs in super enhancers was found in a cell line derived from xenografted liver metastasis but not the parental cell line. The prediction method was further extended to pancreatic cancer where liver metastasis is also a common finding, and a similar enrichment of HNF1 family motif in super-enhancers was found. We subsequently showed that HNF1A was prominently expressed and significantly upregulated in metastatic cell lines and clinical specimens of liver metastases. Collectively, our study implicates HNF1A as a master transcription factor involved in shaping global super-enhancer landscape in CRC liver metastasis.
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| Overall design |
Examination of H3K27ac profile in KM12SM and parental KM12C cells
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| Contributor(s) |
Cai C, Bi D, Liu H, Qin H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 16, 2020 |
| Last update date |
Oct 05, 2021 |
| Contact name |
Huanlong Qin |
| Organization name |
Shanghai Tenth People’s Hospital Affiliated to Tongji University
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| Department |
Gastrointestinal Surgery
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| Street address |
301 Yan Chang Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200072 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE185332 |
Super-enhancer landscape shaped by master regulator in liver metastasis of colorectal cancer |
|
| Relations |
| BioProject |
PRJNA678710 |
| SRA |
SRP292737 |
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