|
| Status |
Public on Dec 31, 2020 |
| Title |
Single-cell transcriptomic analysis of the adult mouse spinal cord reveals fundamental molecular diversity of autonomic and skeletal motor neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To characterize the diversity of cholinergic neurons in the spinal cord, we performed single-nucleus RNA sequencing on cholinergic-enriched pools of nuclei from the adult mouse.
|
| |
|
| Overall design |
Enrichment: Nuclei were enriched by fluorescence-activated nuclei sorting (FANS) of GFP+ cholinergic nuclei from reporter mice.
Barcoding: Nuclei were loaded into a 10X Chromium device and barcoded via oligo-dT reverse transcription using the V3 chemistry. Library prep: Libraries were prepared by PCR and size-exclusion according to published 10X protocols. Sequencing: Libraries were sequenced using NextSeq 550 high-throughput, 150-cycle kits and NovaSeq 100-cycle kits.
|
| |
|
| Contributor(s) |
Blum JA, Klemm S, Shadrach JL, Guttenplan KA, Nakayama L, Kathiria A, Hoang PT, Gautier O, Kaltschmidt JA, Greenleaf WJ, Gitler AD |
| Citation(s) |
33589834 |
| Submission date |
Nov 17, 2020 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Aaron Gitler |
| E-mail(s) |
agitler@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Gitler Lab
|
| Street address |
Beckman Center
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21626 |
NextSeq 550 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA678959 |
| SRA |
SRP292908 |
Less...
More...