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Series GSE161644 Query DataSets for GSE161644
Status Public on May 24, 2021
Title Iron supplementation regulates the progression of high fat diet induced obesity and hepatic steatosis via mitochondrial signaling pathways [I]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Disruption of iron metabolism is closely related to metabolic diseases. Iron deficiency is frequently associated with obesity and hepatic steatosis. However, the effects of iron supplementation on obesity and energy metabolism remain unclear. Here we show that a high-fat diet supplemented with iron reduces body weight gain and hepatic lipid accumulation in mice. Iron supplementation was found to reduce mitochondrial morphological abnormalities and upregulate gene transcription involved in mitochondrial function and beta oxidation in the liver and skeletal muscle. In both these tissues, iron supplementation increased the expression of genes involved in heme or iron–sulfur (Fe–S) cluster synthesis. Heme and Fe–S cluster, which are iron prosthetic groups contained in electron transport chain complex subunits, are essential for mitochondrial respiration. The findings of this study demonstrated that iron regulates mitochondrial signaling pathways—gene transcription of mitochondrial component molecules synthesis and their energy metabolism. Overall, the study elucidates the molecular basis underlying the relationship between iron supplementation and obesity and hepatic steatosis progression, and the role of iron as a signaling molecule.
 
Overall design Male C57BL/6J mice of 6 weeks of age were fed with a control diet (Control;10% kcal fat), a high-fat diet (HF; 60% kcal fat), or a high-fat diet supplemented with 0.023% (w/w) sodium ferrous citrate (HF+SFC) for 15 weeks. Control diet (D12450B) contained 10 kcal% fat, 20 kcal% protein, and 70 kcal% carbohydrate, whereas the HF diet (D12492) contained 60 kcal% fat, 20 kcal% protein, and 20 kcal% carbohydrate (Research Diets, Inc., New Brunswick, NJ, USA). For each group, a mix of all sample cDNAs was used.
 
Contributor(s) Kitamura N, Yokoyama Y, Taoka H, Nagano U, Hosoda S, Taworntawat T, Nakamura A, Ogawa Y, Tsubota K, Watanabe M
Citation(s) 34031430
Submission date Nov 17, 2020
Last update date Jun 03, 2021
Contact name Naho Kitamura
E-mail(s) nahoshi@sfc.keio.ac.jp
Organization name Keio University
Department Graduate School of Media and Governance
Street address 5322, Endo
City Fujisawa
State/province Kanagawa
ZIP/Postal code 252-0882
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (3)
GSM4911751 Liver_Control_1
GSM4911752 Liver_HF_1
GSM4911753 Liver_HF+SFC_1
Relations
BioProject PRJNA678988

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161644_RAW.tar 10.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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