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Series GSE16174 Query DataSets for GSE16174
Status Public on Oct 12, 2009
Title Reticulocyte 3 timepoints
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse models have proven invaluable for understanding erythropoiesis. Here, we describe an autosomal recessive inherited anemia in the mouse mutant hem6. Hematologic and transplantation analyses revealed a mild, congenital, hypochromic, microcytic anemia intrinsic to the hematopoietic system that is associated with a decreased red blood cell zinc protoporphyrin to heme ratio, indicative of porphyrin insufficiency. Iron uptake experiments showed that hem6 reticulocytes are defective in heme production, but not cellular iron uptake defects. Male hem6 mice are infertile due to defects in sperm structure and motility. Through positional cloning and BAC complementation, we identified the gene responsible for the hem6 anemia. We hypothesized that the relative deficiency in erythroid-specific mRNAs in hem6 reticulocytes might be due to decreased mRNA stability. Indeed, serial microarray analysis of reticulocytes aged in vitro showed that numerous, abundantly expressed erythroid-specific transcripts decayed at faster rates in hem6 reticulocytes compared to control reticulocytes. Furthermore, these mRNAs also have progressively shorter poly (A) tails, suggesting a mechanism for the increased rate of decay.

Keywords: serial time points
 
Overall design Reticulocyte rich blood were collected and cultured ex vivo for 24 hours, samples were collected at 0, 12,24 hours for microarray analysis. There are 3 wild type (wt) biological replicates and 5 mutant (mut) biological replicates in each time point.
 
Contributor(s) Tian M
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 20, 2009
Last update date Jun 14, 2018
Contact name meng tian
E-mail(s) mengtian@fas.harvard.edu
Phone 6179192263
Organization name Children's Hospital Boston
Department Pathology
Lab Fleming
Street address 320 Longwood Ave Enders1110
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM406246 Retic_0_wt_Rep1
GSM406247 Retic_0_wt_Rep2
GSM406248 Retic_0_wt_Rep3
Relations
BioProject PRJNA115061

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16174_RAW.tar 3.1 Mb (http)(custom) TAR
GSE16174_nonorm_nobkgd.txt.gz 7.5 Mb (ftp)(http) TXT
GSE16174_readme.txt 407 b (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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