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Series GSE16178 Query DataSets for GSE16178
Status Public on Jul 29, 2009
Title Induction of Pluripotency in Adult Unipotent Germline Stem Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.
 
Overall design Nine samples were analyzed.
GSC1: Mouse Germ Stem Cells, line 1 (1 replicate), GSC2: Mouse Germ Stem Cells, line 2 (1 replicate), GSC3: Mouse Germ Stem Cells, line 3 (1 replicate), GSC4: Mouse Germ Stem Cells, line 4 (1 replicate)
gPS1: Mouse clonal germ Pluripotent Stem cells, line 1, GFP-sorted (1 replicate), gPS2: Mouse clonal germ Pluripotent Stem cells, line 2, GFP-sorted (1 replicate), gPS3: Mouse clonal germ Pluripotent Stem cells, line 3, GFP-sorted (1 replicate)
ESC: Mouse Embryonic Stem Cells (duplicate)
 
Contributor(s) Ko K, Tapia N, Kim J, Araúzo-Bravo MJ, Sasse P, Glaser T, Wu G, Ruau D, Han D, Greber B, Hausdörfer K, Sebastiano V, Stehling M, Fleischmann BK, Brüstle O, Zenke M, Schöler HR
Citation(s) 19570517
Submission date May 20, 2009
Last update date Jun 14, 2018
Contact name Marcos J Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM406304 GSC line 1
GSM406305 GSC line 2
GSM406306 GSC line 3
Relations
BioProject PRJNA115067

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16178_RAW.tar 3.1 Mb (http)(custom) TAR
GSE16178_non-normalized_data.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Raw data provided as supplementary file

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