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| Status |
Public on Feb 23, 2021 |
| Title |
Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Drosophila reproductive behaviors are directed by fruitless neurons. A reanalysis of genomic studies shows that genes encoding dpr and DIP Immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. We find that each fru P1 and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes indicates that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of fru P1 neurons shows that many DIPs have high expression in a small set of neurons, whereas the dprs are often expressed in a larger set of neurons at intermediate levels, with a myriad of dpr/DIP expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of DIP-ε change the sex-specific morphologies of fru P1 ∩ DIP-α neurons.
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| Overall design |
scRNA-seq using 10x Genomics CHROMIUM Single Cell 3’ Solution V2 Chemistry of 48hr after puparium formation (APF) male Drosophila Central Nervous System (CNS) cells where fru P1-expressing cells were genetically labeled with GFP.
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| Contributor(s) |
Palmateer C, Arbeitman M |
| Citation(s) |
33616528 |
| Submission date |
Nov 24, 2020 |
| Last update date |
Feb 26, 2021 |
| Contact name |
Michelle N Arbeitman |
| E-mail(s) |
michelle.arbeitman@med.fsu.edu
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| Organization name |
Florida State University
|
| Department |
Department of Biomedical Sciences
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| Lab |
Arbeitman Lab
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| Street address |
1115 W. Call Street
|
| City |
Tallahassee |
| State/province |
Florida |
| ZIP/Postal code |
32306 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA680563 |
| SRA |
SRP293941 |
