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| Status |
Public on May 30, 2009 |
| Title |
Effects of heat, anoxia, and combined heat-anoxia treatments |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia, heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis.
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| Overall design |
We treated Arabidopsis seedling, 4-days old, dark germinated with:
-Control (23°C, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-Heat-treated (38°C for 90 minutes, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-Anoxia-treated (23°C, under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
-combined heat+Anoxia-treatment (23°C, treated at 38°C for 90 min and thereafter under anoxia for 6h, dark, liquid Murashige-Skoog medium containing 30mM sucrose).
Two biological replicates for each condition.
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| Contributor(s) |
Perata P, Banti V, Maffessoni F, Loreti E, Alpi A |
| Citation(s) |
20089772 |
| Submission date |
May 26, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Pierdomenico Perata |
| E-mail(s) |
p.perata@sssup.it
|
| Phone |
+39050881901
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| Organization name |
Sant'Anna School of Advanced Studies
|
| Department |
Institute of Life Sciences
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| Lab |
PlantLab
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| Street address |
Via Guidiccioni 8-10
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| City |
San Giuliano Terme |
| State/province |
Pisa |
| ZIP/Postal code |
56010 |
| Country |
Italy |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA117165 |
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