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| Status |
Public on Mar 17, 2021 |
| Title |
Elevated Extracellular cGMP Produced after Exposure to Enterotoxigenic Escherichia coli (ETEC) Heat-Stable Toxin (ST) Induces Epithelial IL-33 Release and Alters Intestinal Immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The purpose of our study was to examine the effect of enterotoxigenic E. coli (ETEC) heat-stable toxin (ST) on global T84 gene expression at selected time points following intoxication, with the goal of understanding the functional effects of ST on host epithelium. RNASeq analysis relvealed that ST alters inflammatory gene expression, including the IL-1 family member IL33, which is induced downstream of cGMP.
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| Overall design |
We studied the effects of ETEC ST on T84 gene expression. We treated T84 cells with ST and collected three replicates each after 3 hr, 6 hr, and 24 hr post-intoxication. Treatment groups (in triplicate) were compared to untreated T84 cells (in triplicate).
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| Contributor(s) |
Motyka NI, Stewart SR, Hollifield IE, Kyllo TR, Mansfield JA, Norton EB, Clements JD, Bitoun JP |
| Citation(s) |
33431701 |
| Submission date |
Dec 01, 2020 |
| Last update date |
Jun 16, 2021 |
| Contact name |
Jay Kolls |
| Organization name |
Tulane University
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| Department |
Medicine
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| Lab |
Center for Translational Research in Infection and Inflammation
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| Street address |
1430 Tulane Ave
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| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA681781 |
| SRA |
SRP295300 |
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