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| Status |
Public on May 31, 2010 |
| Title |
Analysis of cardiac gene expression in response to isoproterenol-induced heart failure (part 1) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Myoglobin knockout mice (myo-/-) adapt to the loss of myoglobin by the activation of a variety of compensatory mechanisms on the structural and functional level. In order to analyze to what extent myo-/- mice would tolerate cardiac stress we used the model of chronic isoproterenol application to induce cardiac hypertrophy in myo-/- mice and wild type (WT) controls. After 14 d of isoproterenol infusion cardiac hypertrophy in WT and myo-/- mice reached a similar level. WT mice developed lung oedema and left ventricular dilatation indicating the development of heart failure. In contrast, myo-/- mice displayed conserved cardiac function and no signs of heart failure. Analysis of the cardiac gene expression profile using 40 k mouse oligonucleotide arrays showed that isoproterenol affected the expression of 180 genes in WT but only 92 genes of myo-/- hearts.
Only 40 of these genes were regulated in WT and myo-/- hearts. Whereas in WT hearts a prononced induction of genes of the extracellular matrix occurred suggesting a higher level of remodelling, in myo-/- hearts genes of carbon metabolism and genes linked to inhibition of apoptosis and muscular repair were altered. Interestingly, a subset of genes which was altered in myo-/- mice already under basal conditions was differentially expressed in WT hearts under isoproterenol treatment. In summary, our data show, that the genetic background (WT, myo-/-) has a major impact on cardiac gene expression even in the context of an aggressive hypertrophy model such as chronic isoproterenol stimulation.
Keywords: gene expression profiling of isoproterenol induced heart failure in WT and myo-/- mice
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| Overall design |
We analysed the cardiac gene expression profiles in a total of 32 hearts subdivided into 4 groups (8 WT vehicle, 8 WT ISO, 8 myo-/- vehicle, 8 myo-/- ISO).
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| Contributor(s) |
Molojavyi A, Lindecke A, Kessels C, Kocks S, Köhrer K, Gödecke A |
| Citation(s) |
20145201 |
| Submission date |
May 27, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Antje Lindecke |
| E-mail(s) |
antje.lindecke@uni-duesseldorf.de
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| Phone |
49-211-8114367
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| Organization name |
Universitaet Duesseldorf
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| Department |
BMFZ
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| Lab |
Microarray
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| Street address |
Universitaetsstr. 1
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| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA115177 |
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