|
| Status |
Public on May 28, 2009 |
| Title |
Gene expression in a Highly Tumorigenic Cells (HTC) purified from Urothelial Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used DNA microarrays to compare RNA transcript expression from distinct cell populations sorted by FACS with an antibody against the 67Kd laminin receptor (67LR), and showing distinct tumorigenicity in vivo.
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| |
|
| Overall design |
Sorted cells were derived by digesting xenografted sw 780 tumors into single cell suspensions. Differential gene expression was assessed by direct comparisons of labeled moieties, using a dye-swap design and biological replication. Hybridizations were performed on Agilent (Santa Clara, CA) Whole Human Genome DNA microarrays (hgug4112a). Three different samples were analyzed: 1) Urothelial cancer cells expressing 67LR (67LR+, BRIGHT); 2) Urothelial cancer cells not expressing 67LR (67LR-, DIM); 3) Control sorted urothelial cancer cells (BULK, containing both BRIGHT and DIM cells). In this series we provide normalized single channel intensities, aligned with the corresponding raw expression data.
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| |
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| Contributor(s) |
Marchionni L |
| Citation(s) |
19544456 |
| Submission date |
May 27, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Luigi Marchionni |
| E-mail(s) |
marchion@jhu.edu
|
| Phone |
410-502-8179
|
| Organization name |
Johns Hopkins University
|
| Department |
Oncology
|
| Lab |
Cancer Biology Program
|
| Street address |
1550 Orleans St., CRB2, Room 1M52
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (16)
|
| GSM409291 |
67LR positive population (jhu-251485011211-1.txt_Cy3) |
| GSM409292 |
67LR positive population (jhu-251485011212-1.txt_Cy5) |
| GSM409293 |
Whole population (jhu-251485011212-2.txt_Cy3) |
| GSM409294 |
67LR negative population (jhu-251485011212-2.txt_Cy5) |
| GSM409295 |
67LR negative population (jhu-251485011212-3.txt_Cy3) |
| GSM409296 |
67LR positive population (jhu-251485011212-3.txt_Cy5) |
| GSM409297 |
67LR positive population (jhu-251485011212-4.txt_Cy3) |
| GSM409298 |
Whole population (jhu-251485011212-4.txt_Cy5) |
| GSM409299 |
67LR negative population (jhu-251485011211-1.txt_Cy5) |
| GSM409300 |
67LR negative population (jhu-251485011211-2.txt_Cy3) |
| GSM409301 |
Whole population (jhu-251485011211-2.txt_Cy5) |
| GSM409302 |
Whole population (jhu-251485011211-3.txt_Cy3) |
| GSM409303 |
67LR positive population (jhu-251485011211-3.txt_Cy5) |
| GSM409304 |
Whole population (jhu-251485011211-4.txt_Cy3) |
| GSM409305 |
67LR positive population (jhu-251485011211-4.txt_Cy5) |
| GSM409306 |
Whole population (jhu-251485011212-1.txt_Cy3) |
|
| Relations |
| BioProject |
PRJNA115089 |
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