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Series GSE163085 Query DataSets for GSE163085
Status Public on Apr 13, 2021
Title Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2 [cell lines]
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis.
Overall design We studied the effect of Brn2 loss on in vivo melanomagenesis by generating the mouse cell lines (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2+/+) = (Braf-Pten-Brn2-wt), (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/+) = (Braf-Pten-Brn2-het) and (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/F) = (Braf-Pten-Brn2-hom) by introducing the floxed Brn2 locus into the genome by appropriate crossings (Figure 2 S2A) {Jaegle, 2003 #1672}. Cre-mediated defloxing of Braf, Pten, and Brn2 loci was induced by topical application of tamoxifen during the first three days after birth (Figure 2 S2B). All mice were monitored for the number of tumors/mice, and the growth rate of the first tumor. Cell lines were established from these tumors
Contributor(s) Hamm M, Sohier P, Raymond JH, Larue L
Citation(s) 34140478
Submission date Dec 12, 2020
Last update date Jul 08, 2021
Contact name Lionel Larue
Phone +33 1 69 86 71 07
Organization name Institut Curie
Department UMR3347 CNRS - U1021 INSERM, Institut Curie
Lab Normal and Pathological Development of Melanocytes
Street address Bat 110, Centre universitaire
City Orsay
ZIP/Postal code 91405
Country France
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM4971819 Cell_line_Braf-Pten-Brn2-wt_1
GSM4971820 Cell_line_Braf-Pten-Brn2-wt_2
GSM4971821 Cell_line_Braf-Pten-Brn2-wt_3
This SubSeries is part of SuperSeries:
GSE163086 Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2
BioProject PRJNA684751

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Supplementary file Size Download File type/resource
GSE163085_RAW.tar 31.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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