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Series GSE163085 Query DataSets for GSE163085
Status Public on Apr 13, 2021
Title Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2 [cell lines]
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis.
 
Overall design We studied the effect of Brn2 loss on in vivo melanomagenesis by generating the mouse cell lines (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2+/+) = (Braf-Pten-Brn2-wt), (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/+) = (Braf-Pten-Brn2-het) and (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/F) = (Braf-Pten-Brn2-hom) by introducing the floxed Brn2 locus into the genome by appropriate crossings (Figure 2 S2A) {Jaegle, 2003 #1672}. Cre-mediated defloxing of Braf, Pten, and Brn2 loci was induced by topical application of tamoxifen during the first three days after birth (Figure 2 S2B). All mice were monitored for the number of tumors/mice, and the growth rate of the first tumor. Cell lines were established from these tumors
 
Contributor(s) Hamm M, Sohier P, Raymond JH, Larue L
Citation(s) 34140478
Submission date Dec 12, 2020
Last update date Jul 08, 2021
Contact name Lionel Larue
Phone +33 1 69 86 71 07
Organization name Institut Curie
Department UMR3347 CNRS - U1021 INSERM, Institut Curie
Lab Normal and Pathological Development of Melanocytes
Street address Bat 110, Centre universitaire
City Orsay
ZIP/Postal code 91405
Country France
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM4971819 Cell_line_Braf-Pten-Brn2-wt_1
GSM4971820 Cell_line_Braf-Pten-Brn2-wt_2
GSM4971821 Cell_line_Braf-Pten-Brn2-wt_3
This SubSeries is part of SuperSeries:
GSE163086 Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2
Relations
BioProject PRJNA684751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE163085_RAW.tar 31.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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