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| Status |
Public on Jun 18, 2021 |
| Title |
RNA-Seq analysis SIRT1 overexpression effect on pancreatic tumor tissue in NOD/SCID mice |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pancreatic cancer is a common malignant tumor with poor prognosis. Accumulating evidence indicates that sirtuin 1 (SIRT1) exerts biological functions in various cancers.SIRT1 plays an important role in tumorigenesis, development, and drug resistance by blocking aging and apoptosis, also promoting cell growth and angiogenesis.However, how SIRT1 functions in the development of pancreatic cancer stem cells (CSCs) is currently poorly understood. Here, we showed that SIRT1 inhibits the differentiation of cancer cells by inhibiting various genes expression , including GRHL3, thus promoting the stemness and tumorigenesis of pancreatic cancer.
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| Overall design |
PANC-1 cells transfected with stable expression of vector or SIRT1. These cells were injected subcutaneously into the groin of 6-week-old female NOD/SCID mice under limiting dilutions. We further extracted tota RNA using Trizol reagent (Invitrogen, USA) from tumor samples 4 weeks after injection. Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.
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| Contributor(s) |
Leng S, Wang Y |
| Citation(s) |
34163012 |
| Submission date |
Dec 13, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
yan wang |
| E-mail(s) |
yanwang@tmu.edu.cn
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| Organization name |
National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences
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| Street address |
17 panjiayuan nanli, chaoyang district, Beijing, China
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| City |
beijing |
| ZIP/Postal code |
100021 |
| Country |
Zimbabwe |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA684905 |
| SRA |
SRP297790 |
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