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| Status |
Public on Feb 23, 2021 |
| Title |
Mapping of p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In the article "Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers" by Bejjani et al., we mapped p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1) to study whether Fra-1 can modulate p300/CBP recruitment on MDA-MB-231 genome
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| Overall design |
MDA-MB-231 cells were transfected with a control siRNA or a siRNA directed against Fra-1. ChIP-seq were carried out using an mouse monoclonal antibody specific of p300/CBP (sc-32244X from Santa Cruz Biotechnology)
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| Web link |
http://0-doi-org.brum.beds.ac.uk/10.1093/nar/gkab053
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| Contributor(s) |
Bejjani F, Piechaczyk M, Jariel-Encontre I |
| Citation(s) |
33533919 |
| Submission date |
Dec 16, 2020 |
| Last update date |
Apr 06, 2021 |
| Contact name |
Isabelle Jariel-Encontre |
| E-mail(s) |
isabelle.jariel@inserm.fr
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| Phone |
00 33 (0)4 11 28 31 53
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| Organization name |
IRCM-U1194- INSERM
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| Street address |
208 Avenue des Apothicaires
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| City |
Montpellier |
| State/province |
Montpellier Cedex5 |
| ZIP/Postal code |
34298 |
| Country |
France |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
| GSM4976879 |
MDA-MB-231: Input |
| GSM4976880 |
MDA-MB-231: p300/CBP in cells transfected with control siRNA |
| GSM4976881 |
MDA-MB-231: p300/CBP in cells transfected with siRNA directed against Fra-1 |
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| Relations |
| BioProject |
PRJNA685732 |
| SRA |
SRP298101 |
