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Series GSE163875 Query DataSets for GSE163875
Status Public on Dec 27, 2020
Title Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells
Organisms Bombina orientalis; Xenopus laevis
Experiment type Expression profiling by high throughput sequencing
Summary Exposure to particulate matter (PM) in ambient air is known to increase the risk of cardiovascular disorders and mortality. The cytotoxicity of PM is mainly due to the abnormal increase of reactive oxygen species (ROS), which damage cellular components such as DNA, RNA, and proteins. The correlation between PM exposure and human disorders, including mortality, is based on long-term exposure. In this study we have investigated acute responses of mucus-secreting goblet cells upon exposure to PM derived from a heavy diesel engine. To this end, we employed the mucociliary epithelium of amphibian embryos and human Calu-3 cells to examine PM mucotoxicity. Our data suggest that acute exposure to PM significantly impairs mucus secretion and results in the accumulation of mucus vesicles in the cytoplasm of goblet cells. RNA-seq analysis revealed that acute responses to PM exposure significantly altered gene expression patterns; however, known regulators of mucus production and the secretory pathway were not significantly altered. Interestingly, pretreatment with α-tocopherol nearly recovered the hyposecretion of mucus from both amphibian and human goblet cells. We believe this study demonstrates the mucotoxicity of PM and the protective function of α-tocopherol on mucotoxicity caused by acute PM exposure from heavy diesel engines.
 
Overall design For each species (X. laevis and B. orientalis), we treated 100 ug/mL of the particulate matters (PM) to whole embryo. For each condition, we used duplicated biological replicates. To evaluate the transcriptonal response among time, we sampled at two time points: 3 hours after PM treatment, and 9 hours after the treatment.
 
Contributor(s) Kwon T, Yang H, Sim HJ, Cho H, Bang WY, Kim HE, Kwon TK, Park TJ
Citation(s) 32277121
Submission date Dec 26, 2020
Last update date Oct 17, 2023
Contact name Taejoon Kwon
E-mail(s) tkwon@unist.ac.kr
Organization name Ulsan National Institute of Science and Technology
Department Department of Biomedical Engineering
Street address 50 Unist-gil
City Ulsan
ZIP/Postal code 44919
Country South Korea
 
Platforms (2)
GPL18936 Illumina HiSeq 2500 (Xenopus laevis)
GPL26992 Illumina HiSeq 2500 (Bombina orientalis)
Samples (16)
GSM4988988 B. orientalis embryo, 3hr, control, rep 1
GSM4988989 B. orientalis embryo, 3hr, control, rep 2
GSM4988990 B. orientalis embryo, 9hr, control, rep 1
Relations
BioProject PRJNA687987
SRA SRP299616

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE163875_BOMOR+XENLA+PM.kallisto.zip.gz 11.1 Mb (ftp)(http) ZIP
GSE163875_Bombina_orientalis.a2020_08.cdna.fa.gz 7.9 Mb (ftp)(http) FA
GSE163875_XENLA_XB201901_tx_annot.fa.gz 44.1 Mb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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