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| Status |
Public on Jan 13, 2021 |
| Title |
Differences in TCR repertoire and T cell activation underlie the divergent outcomes of anti-tumor immune responses in tumor-eradicating versus tumor-progressing hosts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Background: Anti-tumor immunity is highly heterogeneous between individuals; however, underlying mechanisms remain elusive, despite their potential to improve personalized cancer immunotherapy. Head and neck squamous cell carcinomas (HNSCCs) vary significantly in immune infiltration and therapeutic responses between patients, demanding a mouse model with appropriate heterogeneity to investigate mechanistic differences.
Methods: We developed a unique HNSCC mouse model to investigate underlying mechanisms of heterogeneous anti-tumor immunity. This model system may provide a better control for tumor-intrinsic and host-genetic variables, thereby uncovering the contribution of the adaptive immunity to tumor eradication. We employed single-cell T-cell receptor (TCR) sequencing coupled with single-cell RNA sequencing to identify the difference in TCR repertoire of CD8 tumor infiltrating lymphocytes (TILs) and the unique activation states linked with different TCR clonotypes.
Results: We discovered that genetically identical wild-type recipient mice responded heterogeneously to the same SCC tumors orthotopically transplanted into the buccal mucosa. While tumors initially grew in 100% of recipients and most developed aggressive tumors, ~25% of recipients reproducibly eradicated tumors without intervention. Heterogeneous anti-tumor responses were dependent on CD8 T cells. Consistently, CD8 TILs in regressing tumors were significantly increased and more activated. Single-cell TCR-sequencing revealed that CD8 TILs from both growing and regressing tumors displayed evidence of clonal expansion compared with splenic controls. However, top TCR clonotypes and TCR specificity groups appear to be mutually exclusive between regressing and growing TILs. Furthermore, many TCRa/TCRb sequences only occur in one recipient. By coupling single-cell transcriptomic analysis with unique TCR clonotypes, we found that top TCR clonotypes clustered in distinct activation states in regressing vs. growing TILs. Intriguingly, the few TCR clonotypes shared between regressors and progressors differed greatly in their activation states, suggesting a more dominant influence from tumor microenvironment than TCR itself on T cell activation status.
Conclusions: We reveal that intrinsic differences in the TCR repertoire of TILs and their different transcriptional trajectories may underlie the heterogeneous anti-tumor immune responses in different hosts. We suggest that anti-tumor immune responses are highly individualized and different hosts employ different TCR specificities against the same tumors, which may have important implications for developing personalized cancer immunotherapy.
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| Overall design |
(1) We present sequencing data of mouse tumor-infiltrating T cells (TIL) and splenic T cells sequenced by single-cell RNA sequencing in a mouse model of squamous cell carcinoma, A223. Cells from 17 samples (9 expression and 8 VDJ) were sequenced using the 10x genomics platform.
(2) We present sequencing data of the mouse tumor model of squamous cell carcinoma, A223. Samples include whole-exome sequencing and RNA-sequencing of A223 tumor cells grown in vitro.
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| Contributor(s) |
Woolaver RA, Wang JH |
| Citation(s) |
33414263 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 DE027854 |
Mechanisms underlying therapy failure of immune checkpoint inhibitors and Notch1 loss-mediated immunosuppression in head and neck cancers |
UNIVERSITY OF COLORADO DENVER |
Rachel A Woolaver |
| R01 DE027329 |
Elucidating Mechanism of Immune Evasion in Head and Neck Cancers |
UNIVERSITY OF COLORADO DENVER |
Jing Hong Wang |
| R01 DE028420 |
Mechanisms of dual inhibition of TGFbeta/PD-L1 in HNSCC |
UNIVERSITY OF COLORADO DENVER |
Jing Hong Wang |
|
| Submission date |
Jan 03, 2021 |
| Last update date |
Jan 15, 2021 |
| Contact name |
Jing Hong Wang |
| E-mail(s) |
jing.wang@cuanschutz.edu
|
| Phone |
3037248673
|
| Organization name |
University of Colorado at Anschutz
|
| Department |
Immunology and Microbiology
|
| Lab |
Jing H Wang
|
| Street address |
12800 East 19th Ave, Mail stop 8333, RC1 North, Room 9400C
|
| City |
Aurora |
| State/province |
Colorado |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (2) |
| GPL23479 |
BGISEQ-500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA689299 |
| SRA |
SRP300083 |
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