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Series GSE16471 Query DataSets for GSE16471
Status Public on Mar 18, 2010
Title Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The goal of this experiment was to identify the early target genes of constitutively activated CPK5ac and CPK11ac in Arabidopsis mesophyll cells that are involved in early flagellin responses important for plant innate immunity.
We used the microarrays to detail the global program of gene expression underlying initial calcium responses to microbial associated molecualr patterns (MAMPs).
 
Overall design Arabidopsis mesophyll protoplasts were transfected with plasmid DNA to express constitutively active CPK5ac or CPK11ac for 6 hr. Transcript profiles were analyzed and compared between cells transfected with control DNA and CPK5ac or CPK11ac DNA to identify early CPK5ac and CPK11ac target genes.
Web link http://www.nature.com.ezp-prod1.hul.harvard.edu/nature/journal/v464/n7287/suppinfo/nature08794.html
 
Contributor(s) Sheen J, Willmann M, Boudsocq M, Lee H, McCormack M
Citation(s) 20164835
Submission date Jun 06, 2009
Last update date Aug 15, 2018
Contact name Jen Sheen
E-mail(s) sheen@molbio.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Sheen
Street address 185 Cambridge st. CPZN7620
City Boston
State/province MA
ZIP/Postal code 02114-2790
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM413936 protoplasts at T0, biological rep1 [CPK]
GSM413937 protoplasts at T1, biological rep1 [CPK]
GSM413938 protoplasts at T2, biological rep1 [CPK]
This SubSeries is part of SuperSeries:
GSE16557 Gene expression changes underlying initial cellular and molecular responses to MAMPs in Arabidopsis mesophyll cells
Relations
BioProject PRJNA122903

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16471_RAW.tar 13.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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