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| Status |
Public on Mar 18, 2010 |
| Title |
Identification of early flg22 responsive genes in Arabidopsis mesophyll cells |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity.
We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs).
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| Overall design |
Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.
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| Contributor(s) |
Sheen J, Lee H, McCormack M |
| Citation(s) |
20164835 |
| Submission date |
Jun 06, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Jen Sheen |
| E-mail(s) |
sheen@molbio.mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Sheen
|
| Street address |
185 Cambridge st. CPZN7620
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM413942 |
protoplasts at T0, biological rep1 [flg22] |
| GSM413943 |
protoplasts at T1, biological rep1 [flg22] |
| GSM413944 |
protoplasts at T2, biological rep1 [flg22] |
| GSM413945 |
protoplasts at T0, biological rep2 [flg22] |
| GSM413946 |
protoplasts at T1, biological rep2 [flg22] |
| GSM413947 |
protoplasts at T2, biological rep2 [flg22] |
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| This SubSeries is part of SuperSeries: |
| GSE16557 |
Gene expression changes underlying initial cellular and molecular responses to MAMPs in Arabidopsis mesophyll cells |
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| Relations |
| BioProject |
PRJNA122887 |
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