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Series GSE16510 Query DataSets for GSE16510
Status Public on Jun 09, 2009
Title Normal lung transcriptome distinguishes mouse lines with different susceptibility to inflammation and to tumorigenesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary AIRmax and AIRmin mouse lines show a differential lung inflammatory response and differential lung tumor susceptibility after urethane treatment, thus constituting a good genetic model to investigate differences in gene expression profiles related to inflammatory response and lung tumor susceptibility. The transcript profile of ~24,000 known genes was analyzed in normal lung tissue of untreated and urethane-treated AIRmax and AIRmin mice. In lungs of untreated mice, inflammation associated genes involved in pathways such as “leukocyte transendothelial migration”, “cell adhesion” and “tight junctions” were differentially expressed in AIRmax versus AIRmin mice. Moreover, gene expression levels differed significantly in urethane-treated mice even at 21 days after treatment. In AIRmin mice, modulation of expression of genes involved in pathways associated with inflammatory response paralleled the previously observed persistent infiltration of inflammatory cells in the lung of these mice. In conclusion, a specific gene expression profile in normal lung tissue is associated with mouse line susceptibility or resistance to lung tumorigenesis and with different inflammatory response, and urethane treatment causes a long-lasting alteration of the lung gene expression profile that correlates with persistent inflammatory response of AIRmin mice.
Overall design Six AIRmax (3 males and 3 females) and six AIRmin (3 males and 3 females) mice were treated with two intraperitoneal injections of 1 mg/g body weight urethane or saline solution (total of 24 AIR mice: 12 AIRmax and 12 AIRmin mice, 6 urethane- and 6 saline-treated respectively) with a 48-h interval and sacrificed 21 days later
Contributor(s) De Cecco L, Colombo F
Citation(s) 20189714
Submission date Jun 09, 2009
Last update date Jun 14, 2018
Contact name Loris De Cecco
Organization name IRCSS Istituto Nazionale Tumori
Street address via Venezian 1
City Milan
ZIP/Postal code 20133
Country Italy
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM414785 lung_AIRmax_male_urethane_mice2
GSM414786 lung_AIRmin_male_saline_mice19
GSM414787 lung_AIRmin_male_urethane_mice14
BioProject PRJNA116065

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16510_RAW.tar 3.1 Mb (http)(custom) TAR
GSE16510_non-normalized_data.txt.gz 3.9 Mb (ftp)(http) TXT
GSE16510_normalized_data_filtered.txt.gz 1.2 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record
Raw data provided as supplementary file
Processed data provided as supplementary file

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