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| Status |
Public on Jan 23, 2021 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of IgG or IgE producing B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Emergence of IgE antibodies that bind allergens with high affinity is highly correlated with the severity of anaphylaxis. However, the underlying molecular mechanisms by which high-affinity IgE is generated remain poorly understood. We examined the effects of cytokines in IgE class switch using in-vitro B cell activation system
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| Overall design |
B cells from spleen of IgE reporter mice (Verigem) were cultured on the CD154+ fibroblast cell line expressing CD40L and BAFF, NB21.2D9 feeder cells in the presence of recombinant murine IL-4, or IL-21 and/or IL-13. IgG+ or IgE reporter positive B cells were sorted and analyzed the transcriptome using Illumina HiSeq1500.
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| Contributor(s) |
Kubo M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 22, 2021 |
| Last update date |
Jan 25, 2021 |
| Contact name |
Kosuke Miyauchi |
| E-mail(s) |
kosuke.miyauchi@riken.jp
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| Organization name |
RIKEN Center for Integrative Medical Sciences
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| Street address |
1-7-22 Suehiro-sho
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| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA694044 |
| SRA |
SRP302842 |
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