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| Status |
Public on Jan 26, 2021 |
| Title |
scRNAseq of MMTV-Neu primary site and lung cancer cells in early and late stage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cancer cells can disseminate from early-evolved primary lesions. It is thought that a state of early disseminated cancer cell (early DCC) dormancy would precede genetic maturation of DCCs and metastasis initiation. Here we reveal at single cell resolution a previously unrecognized role of mesenchymal- and pluripotency-like programs in coordinating early cancer cell spread and a long-lived dormancy program in early DCCs. We identify in early lesions and early DCCs, the transcription factor ZFP281 as an inducer of mesenchymal- and primed pluripotency-like programs, which is absent in advanced primary tumors and overt metastasis. ZFP281 not only controls the early spread of cancer cells but also locks early DCCs in a prolonged dormancy state by preventing the acquisition of an epithelial-like proliferative program. Thus, ZFP281-driven dormancy of early DCCs may be a rate-limiting step in metastatic progression functioning as a first barrier that DCCs must overcome to then undergo genetic maturation.
This data set aims for a general characterization of the MMTV-Neu mouse model (primary site and lung DCCs) in early and late stages of cancer progression.
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| Overall design |
Single cell RNA profile of early and late breast cancer cells of MMTV-neu mice in the primary site (early lesions, EL; primary tumors, PT) and lungs (early lung disseminated cancer cells, eL DCCs; late lung DCCs, LL DDCs)
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| Web link |
https://www.researchsquare.com/article/rs-145308/v1
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| |
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| Contributor(s) |
Kenigsberg E, Nobre AR |
| Citation missing |
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| Submission date |
Jan 25, 2021 |
| Last update date |
May 05, 2021 |
| Contact name |
Julio Aguirre-Ghiso |
| E-mail(s) |
julio.aguirre-ghiso@mssm.edu
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| Organization name |
Icahn School of Medicine at Mount Sinai
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| Street address |
1468 Madison Avenue, A24-64e
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA694640 |
| SRA |
SRP303156 |
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