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Status |
Public on Sep 19, 2021 |
Title |
Time series (T= 0-4 hours) for E. coli K-12 MC4100 after a shift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth) |
Organism |
Escherichia coli |
Experiment type |
Expression profiling by array
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Summary |
We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. Some are only induced transiently, while are required for long-term adaptation. Many of these genes are related to metabolism, stress response, and immune evasion suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.
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Overall design |
An aerobic, mid-exponential phase (A600= 0.2-0.6) culture was grown at 23˚C in M9 minimal medium containing glycerol as a carbon source. Aliquots of the initial culture were shifted to 37˚C to allow harvest of cells at time points after a shift to 37˚C. Microarray results compare each timepoint after the shift to 37˚C (T= 0.5, 1, or 4 hours) to the initial T=0 timepoint grown at 23˚C.
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Contributor(s) |
White-Ziegler CA |
Citation(s) |
34516284 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R15 GM093908 |
Short-term responses to temperature changes in Escherichia coli |
SMITH COLLEGE |
CHRISTINE A WHITE-ZIEGLER |
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Submission date |
Jan 29, 2021 |
Last update date |
Nov 18, 2021 |
Contact name |
Christine Ann White-Ziegler |
E-mail(s) |
cwhitezi@smith.edu
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Phone |
413-585-3815
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Organization name |
Smith College
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Department |
Biological Sciences
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Lab |
White-Ziegler
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Street address |
Clark Science Center
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City |
Northampton |
State/province |
MA |
ZIP/Postal code |
01063 |
Country |
USA |
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Platforms (1) |
GPL6540 |
University of Alberta MAF Operon E. coli Genome Oligo Set Version 1.0 |
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Samples (9)
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Relations |
BioProject |
PRJNA697923 |