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Series GSE165794 Query DataSets for GSE165794
Status Public on Sep 19, 2021
Title Time series (T= 0-4 hours) for E. coli K-12 MC4100 after a shift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)
Organism Escherichia coli
Experiment type Expression profiling by array
Summary We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. Some are only induced transiently, while are required for long-term adaptation. Many of these genes are related to metabolism, stress response, and immune evasion suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.
 
Overall design An aerobic, mid-exponential phase (A600= 0.2-0.6) culture was grown at 23˚C in M9 minimal medium containing glycerol as a carbon source. Aliquots of the initial culture were shifted to 37˚C to allow harvest of cells at time points after a shift to 37˚C. Microarray results compare each timepoint after the shift to 37˚C (T= 0.5, 1, or 4 hours) to the initial T=0 timepoint grown at 23˚C.
 
Contributor(s) White-Ziegler CA
Citation(s) 34516284
NIH grant(s)
Grant ID Grant title Affiliation Name
R15 GM093908 Short-term responses to temperature changes in Escherichia coli SMITH COLLEGE CHRISTINE A WHITE-ZIEGLER
Submission date Jan 29, 2021
Last update date Nov 18, 2021
Contact name Christine Ann White-Ziegler
E-mail(s) cwhitezi@smith.edu
Phone 413-585-3815
Organization name Smith College
Department Biological Sciences
Lab White-Ziegler
Street address Clark Science Center
City Northampton
State/province MA
ZIP/Postal code 01063
Country USA
 
Platforms (1)
GPL6540 University of Alberta MAF Operon E. coli Genome Oligo Set Version 1.0
Samples (9)
GSM5050080 T=0.5 hours at 37˚C_RepD
GSM5050081 T=0.5 hours at 37˚C_RepE
GSM5050082 T=0.5 hours at 37˚C_RepH
Relations
BioProject PRJNA697923

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165794_RAW.tar 22.3 Mb (http)(custom) TAR (of GPR)
GSE165794_processed_data.xlsx 1.9 Mb (ftp)(http) XLSX
Processed data are available on Series record

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