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| Status |
Public on Feb 05, 2021 |
| Title |
scRNA-Seq of peripheral CD8 T cells from patients with metastatic melanoma taking ICI therapy. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Patients with metastatic melanoma have been treated with the new class of drug named immune checkpoint inhibitors. Single-cell RNA-sequencing was performed in circulating CD8+ T cells isolated by cell sorting from 20 patients who were followed for 6 months. Among them, 11 responded to therapy, whilst 9 did not. Overall, the data obtained by sc-RNA seq showed the presence of MAIT cells, that had higher levels in those who showed a better response to treatment.
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| Overall design |
scRNA-Seq of peripheral CD8 T cells obtained from 20 melanoma patients (11 Responders and 9 Non-Responders) before (T0), after first (T1) and second (T2) cycle of ICI therapy. Almost all samples were sequenced in four replicates.
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| Contributor(s) |
Lo Tartaro D, Forcato M, Bicciato S, Cossarizza A |
| Citation(s) |
33723257 |
| Submission date |
Feb 04, 2021 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Andrea Cossarizza |
| E-mail(s) |
andrea.cossarizza@unimore.it
|
| Organization name |
University of Modena and Reggio Emilia
|
| Street address |
Via Campi 287
|
| City |
Modena |
| State/province |
Modena |
| ZIP/Postal code |
41125 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (183)
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| Relations |
| BioProject |
PRJNA699546 |
| SRA |
SRP304741 |
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