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Series GSE166194 Query DataSets for GSE166194
Status Public on Mar 05, 2021
Title The Genome-Wide Binding Landscape of Estrogen Receptor β in Human Colon Can-cer Cells Reveals Its Tumor Suppressor Activity
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In order to elucidate the mechanism of ERβ in the colon, we validated ERβ antibodies, optimized chromatin-immunoprecipitation (ChIP), and performed ChIP-Seq of CRC engineered cell lines re-expressing ERβ. In addition, we also performed H3K27AC ChIP-Seq to analyse histone marks in presence of ERβ. We present for the first time, the cistrome of nuclear receptor ERβ in different CRC cell lines and corresponding H3K27AC histone marks.
 
Overall design Each ChIP was performed three times. For further analysis, we focused on sites present in at least two out of three replicates
 
Contributor(s) Indukuri R, Williams C
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Submission date Feb 04, 2021
Last update date Mar 08, 2021
Contact name Rajitha Indukuri
E-mail(s) rajitha.indukuri@scilifelab.se
Phone 0760285999
Organization name Scilifelab
Street address Tomtebodavägen 23a, Solna
City Stockholm
State/province Sweden
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (10)
GSM5065666 SW480M H3K27AC ChIP-Seq rep 1
GSM5065667 SW480M H3K27AC ChIP-Seq rep 2
GSM5065668 SW480ERβ H3K27AC ChIP-Seq rep 1
Relations
BioProject PRJNA699567
SRA SRP304761

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166194_RAW.tar 271.4 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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