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Series GSE166243 Query DataSets for GSE166243
Status Public on Feb 06, 2021
Title NAP1L2 Drives Mesenchymal Stem Cell Senescence and Suppresses Osteogenic Differentiation [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Changes in these genes are probably a consequence of aging, and the real regulators governing BMSC senescence and osteogenesis are still unclear.In the current study, we report that nucleosome assembly-related protein NAP1L2 serves as an important regulator of both the senescence and osteogenic differentiation of BMSCs through inhibition of NF-κB signaling and recruitment of SIRT1 to deacetylate the H3K14ac level on promoters of osteogenic genes. Thus, targeting NAP1L2 using an aging antagonist such as NMN would benefit aging-related disease.
 
Overall design Non-target control and knockdown Nap1l2 C3H10T1/2 cells were divided into the following four groups depending on whether osteogenesis induction for 7days is performed: NC, KD-Nap1l2, NC-7day, KD-Nap1l2-7day.And cells were harvested for gene expression profiling. Two replicates were performed.
 
Contributor(s) Hu M
Citation(s) 35032339
Submission date Feb 05, 2021
Last update date Mar 04, 2022
Contact name Liu Zhiqiang
E-mail(s) zhiqiangliu@tmu.edu.cn
Organization name Tianjin Medical University
Street address 22 Qxiangtai Rd
City Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (8)
GSM5066746 Ctrl_0d_1
GSM5066747 Ctrl_0d_2
GSM5066748 Ctrl_7d_1
This SubSeries is part of SuperSeries:
GSE166244 NAP1L2 Drives Mesenchymal Stem Cell Senescence and Suppresses Osteogenic Differentiation
Relations
BioProject PRJNA699765
SRA SRP304879

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166243_gene_diff.txt.txt.gz 641.3 Kb (ftp)(http) TXT
GSE166243_gene_expression.txt.txt.gz 1.1 Mb (ftp)(http) TXT
GSE166243_rna_diff.txt.txt.gz 1.9 Mb (ftp)(http) TXT
GSE166243_rna_expression.txt.txt.gz 3.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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