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| Status |
Public on Feb 09, 2021 |
| Title |
Mesenchymal Stem Cells (MSCs) Alleviate Experimental Immune-mediated Liver Injury via Chitinase 3-like protein 1-mediated T cell suppression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-seq for MSCs from human bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each). RNA-sequencing for human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 μg/mL) for 48 h， and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h.
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| Overall design |
MSCs from bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each) were collected and sequenced to identify the differently expressed genes and related pathways. FACS sorted human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 μg/mL) for 48 h, and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h were collected and sequenced to identify the differently expressed genes and related pathways.
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| Contributor(s) |
Zhang Q, Xu Y, Xiang AP, Liu Q |
| Citation(s) |
33664231 |
| Submission date |
Feb 08, 2021 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Yan Xu |
| E-mail(s) |
xuyan55@mail.sysu.edu.cn
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| Organization name |
The Third Affiliated Hospital of Sun Yat-Sen University
|
| Department |
Biotherapy Center
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| Street address |
No. 600 Tianhe Road
|
| City |
Guangzhou |
| ZIP/Postal code |
510630 |
| Country |
China |
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| Platforms (2) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA700504 |
| SRA |
SRP305274 |
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