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Series GSE166336 Query DataSets for GSE166336
Status Public on Aug 24, 2021
Title Insulin response in mouse liver
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary An effective combination of multi-omic datasets can enhance our understanding of complex biological phenomena. To build a context-dependent network with multiple omic layers, i.e., a trans-omic network, we performed phosphoproteomics, transcriptomics, proteomics, and metabolomics of murine liver for 4 h after insulin administration and integrated the time series. Structural characteristics and dynamic nature of the network were analyzed to elucidate the impact of insulin. Early and prominent changes in protein phosphorylation and persistent and asynchronous changes in mRNA and protein levels through non-transcriptional mechanisms indicate enhanced crosstalk between phosphorylation-mediated signaling and protein expression regulation. Metabolic response shows different temporal regulation with transient increases at early time points across categories and enhanced response in the amino acid and nucleotide categories at later time points due to process convergence. This extensive and dynamic view of the trans-omic network elucidates prominent regulatory mechanisms that drive insulin responses through intricate interlayer coordination.
 
Overall design RNA-seq of mouse liver over a period of four hours after insulin/PBS administration. For each condition (insulin/PBS), data was collected at five time points with biological triplicates (total of thirty samples).
 
Contributor(s) Matsuzaki F, Maehara K, Ohkawa Y, Kubota H
Citation(s) 34433063
BioProject PRJNA679692
Submission date Feb 08, 2021
Last update date Aug 29, 2021
Contact name Fumiko Matsuzaki
E-mail(s) fumu@bioreg.kyushu-u.ac.jp
Organization name Kyushu University
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (30)
GSM5068689 LiverInsulin_1_1
GSM5068690 LiverInsulin_1_2
GSM5068691 LiverInsulin_1_3
Relations
SRA SRP293333

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Supplementary file Size Download File type/resource
GSE166336_fpkm.txt.gz 2.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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