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| Status |
Public on Feb 10, 2021 |
| Title |
Dual role of the unfolded protein response in the therapeutic effect of AZD1775 on TP53 mutant high grade serous ovarian cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
we demonstrate that the WEE1 inhibitor AZD1775 triggers endoplasmic reticulum (ER) stress and activates the PERK and IRE1α branches of the unfolded protein response (UPR) in TP53 mutant HGSOC cells. Upon AZD1775 treatment, PERK facilitates apoptotic signaling in these cells via activating CHOP, whereas IRE1α-induced spliced XBP1 (XBP1s) confers survival in response to WEE1 inhibition. Our data uncover an important dual role of UPR in TP53 mutant HGSOC cells in response to AZD1775, where additional inhibition of IRE1α-XBP1s signaling may offer synergistic efficacy.
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| Overall design |
OVCAR8 cells were treated with DMSO or AZD1775 and the mRNA expression levels were detected by RNA-seq.
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| Contributor(s) |
Xiao R, You L, Xia S, Sun C, Chen G |
| Citation missing |
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| Submission date |
Feb 09, 2021 |
| Last update date |
Feb 10, 2021 |
| Contact name |
Rouou Xiao |
| E-mail(s) |
xiaorou@hust.edu.cn
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| Organization name |
TongJi hospital
|
| Street address |
Wuxian road
|
| City |
Wuhan |
| State/province |
hubei province |
| ZIP/Postal code |
430100 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA700855 |
| SRA |
SRP305471 |
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