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| Status |
Public on Sep 22, 2021 |
| Title |
Mobility of condensin DC is key to its function in repressing transcription [RNA-seq II] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analyze condensin binding by fluorescence recovery after photobleaching (FRAP) and chromatin immunoprecipitation followed by sequencing (ChIP-seq) in ATP hydrolysis mutant and in mutants that affect the level of histone H4K20 methylation and H4K16ac enrichment and depletion on the X chromosome. We have also performed Hi-C analysis to test how 3D DNA interactions mediated by condensin DC change in the catalytic and null mutant of dpy-21, a H4K20me2 demethylase that increase H4K20me1 on the X.
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| Overall design |
Population mRNA profiles of mixed embryos with genotypes: WT, hsp::dpy-27::gfp or hsp::dpy-27[EQ-TR]::gfp.
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| Contributor(s) |
Breimann L, Morao AK, Kim J, David JS, Maryn N, Bikkasani K, Carrozza MJ, Albritton SE, Kramer M, Street LA, Cerimi K, Schumann V, Bahry E, Preibisch S, Woehler A, Ercan S |
| Citation(s) |
34918745 |
| Submission date |
Feb 10, 2021 |
| Last update date |
Dec 22, 2021 |
| Contact name |
Jun Kim |
| E-mail(s) |
kimj50@nyu.edu
|
| Phone |
7183094777
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| Organization name |
NYU
|
| Department |
Biology
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| Lab |
Ercan
|
| Street address |
100 Washington Square East 1009 Silver Center
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE169458 |
Mobility of condensin DC is key to its function in repressing transcription |
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| Relations |
| BioProject |
PRJNA701258 |
| SRA |
SRP305676 |
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