|
| Status |
Public on Feb 01, 2010 |
| Title |
Human keratinocytes exposed to tungsten carbide and tungsten carbide cobalt nanoparticles and cobalt chloride |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparison of transcriptional profiles after exposure of HaCaT cells to WC or WC-Co nanoparticles and respective amount of free cobalt in form of CoCl2 after 3 hours and 3 days of exposure. Genes responding especially to nanoparticles or leached cobalt ions should be determined.
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| Overall design |
Common refernce: pool of 80 RNA Samples of HaCaT cells (treated and not treated), Cy5 labeled. Samples: RNA of HaCaT cells treated 3 hours and 3 days with 30 µg/ml WC nanoparticles, or 33 µg/ml WC-Co nanoparticles (3 µg/ml cobalt content), or 51 µM CoCl2 (equals 3 µg/ml), or water as control, 5 biological replicates for each treatment, all samples were labeled with Cy3 and hybridised together with the common reference.
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| |
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| Contributor(s) |
Busch W |
| Citation(s) |
20105288 |
| Submission date |
Jun 19, 2009 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Wibke Busch |
| E-mail(s) |
wibke.busch@ufz.de
|
| Phone |
00493412351515
|
| Organization name |
UFZ Helmholtz Centre for Environmental Research
|
| Department |
Bioanalytical Ecotoxicology
|
| Lab |
Scholz
|
| Street address |
Permoser Str. 15
|
| City |
Leipzig |
| ZIP/Postal code |
04318 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (40)
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| Relations |
| BioProject |
PRJNA117493 |
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