|
| Status |
Public on Oct 02, 2009 |
| Title |
Translational Changes Upon Dietary Restriction in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of the translational changes induced upon DR in male Drosophila.
|
| |
|
| Overall design |
1-2 day old flies were transferred onto 4% or 0.25% YE food for 6 days. ~100 male flies were then ground and RNA was separated by size on a sucrose gradient and fractionated using a Teledyne density gradient fractionator and a polysome profile was produced by monitoring the abs at 252nm. RNA was then prepared from the low (1-4 ribosomes bound) and high (5 or more ribosomes bound) translation fractions. A translational index was then calculated for each mRNA. The changes in translational index upon DR were then evaluated. This was done in triplicate on individual biological replicates.
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| |
|
| Contributor(s) |
Zid BM, Rogers A, Kapahi P |
| Citation(s) |
19804760 |
| Submission date |
Jun 22, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Brian Matthew Zid |
| E-mail(s) |
zid@ucsd.edu
|
| Organization name |
University of California San Diego
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Zid
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (12)
|
| GSM419413 |
Low Translation Fraction 4% YE |
| GSM419414 |
Low Translation Fraction 4% YE-2 |
| GSM419415 |
Low Translation Fraction 4% YE-3 |
| GSM419416 |
High Translation Fraction 0.25% YE-1 |
| GSM419417 |
High Translation Fraction 0.25% YE-2 |
| GSM419418 |
High Translation Fraction 0.25% YE-3 |
| GSM419419 |
Low Translation Fraction 0.25% YE-1 |
| GSM419420 |
Low Translation Fraction 0.25% YE-2 |
| GSM419421 |
Low Translation Fraction 0.25% YE-3 |
|
| Relations |
| BioProject |
PRJNA117431 |
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