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Series GSE16740 Query DataSets for GSE16740
Status Public on Jun 24, 2009
Title The effect of cardiac troponin T2 knockdown on gene expression in zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary We examined the transcriptional effect of preventing cardiac contraction in zebrafish embryos which can be deprived of circulation without experiencing hypoxia since the fish obtain sufficient oxygen via diffusion. Morpholino antisense knockdown of cardiac troponin T2 (tnnt2) prevented cardiac contraction without affecting vascular development. We concluded that absence of hemodynamic force induces endothelial CXCR4a up-regulation and promotes recovery of blood flow.

We used microarrays to define the genetic signatures of the development of collateral vessels in zebrafish.

Keywords: Time course comparison of wild type zebrafish embryos and morpholino antisense against troponin t2 treated embryos.
 
Overall design One cell zebrafish embryos were injected with morpholino antisense against control or cardiac troponin t2, which prevents cardiac development. Whole embryo RNA was extracted at 36, 48, and 60h post fertilization and transcriptionally profiled.
 
Contributor(s) Packham I, Gray C, Milo M, Chico T
Citation(s) 19509081
Submission date Jun 22, 2009
Last update date Jan 25, 2018
Contact name Marta Milo
E-mail(s) m.milo@sheffield.ac.uk
Organization name University of Sheffield
Department Biomedical Science
Street address Western Bank
City Sheffield
State/province South Yorkshire
ZIP/Postal code S10 2TN
Country United Kingdom
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (18)
GSM419445 RNA from embryos of 3 groups of Wildtype AB zebrafish at 36 hpf_1
GSM419446 RNA from embryos of 3 groups of Wildtype AB zebrafish at 36 hpf_2
GSM419447 RNA from embryos of 3 groups of Wildtype AB zebrafish at 36 hpf_3
Relations
BioProject PRJNA117435

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16740_36h_mmgMOS_DE_genes.xls.gz 1.9 Mb (ftp)(http) XLS
GSE16740_48h_fish_heart_mmgMOS_DE_genes.xls.gz 2.8 Mb (ftp)(http) XLS
GSE16740_60h_fish_heart_mmgMOS_DE_genes.xls.gz 2.3 Mb (ftp)(http) XLS
GSE16740_Ctrl_SiH_time_course_mmgMOS.xls.gz 1.0 Mb (ftp)(http) XLS
GSE16740_DE_genes_time_course_Ctrl.xls.gz 34.9 Kb (ftp)(http) XLS
GSE16740_DE_genes_time_course_SiH.xls.gz 25.3 Kb (ftp)(http) XLS
GSE16740_RAW.tar 29.8 Mb (http)(custom) TAR (of CEL)
GSE16740_readme.txt 2.1 Kb (ftp)(http) TXT
GSE16740_se_time_course.xls.gz 2.9 Mb (ftp)(http) XLS
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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