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Series GSE16858 Query DataSets for GSE16858
Status Public on Dec 24, 2010
Title Human NTERA2 (NT2/D1) cells lines: shZRF1 vs. shRandom during Retinoic Acid (RA) administration
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptional profiling of human NT2 cell lines comparing control cells (shRandom) with ZRF1 knockdown cells (shZRF1) both either untreated (0 h) or treated with Retinoic Acid (3 h) at 0.01 µM. The cell lines were established by retroviral infection allowing for the transcription of either a random shRNA (shRandom) or a shRNA specific for ZRF1 (shZRF1).
The goal was to determine the effect of ZRF1 on transcriptional activation at the onset of differentiation.
 
Overall design Four-condition experiment, shRandom vs. shZRF1 cells either uninduced (0 h) or RA induced (3 h). Biological replicates: 6 replicates (shRandom) taken on 3 different days without or with RA administration. 6 shZRF1 replicates taken on three different days with or without RA administration.
 
Contributor(s) Richly H, Lopez-Bigas N, Di Croce L
Citation(s) 21179169
Submission date Jun 26, 2009
Last update date Jan 23, 2019
Contact name Luciano Di Croce
E-mail(s) luciano.dicroce@crg.eu
Phone +34933160132
Organization name Centre for Genomic Regulation
Street address Dr. Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (12)
GSM422425 shZRF1 vs shRandom 0h Replicate 1
GSM422426 shZRF1 vs shRandom 0h replicate 1 dye swap
GSM422427 shZRF1 vs shRandom 0h Replicate 2
This SubSeries is part of SuperSeries:
GSE16891 Human NTERA2 (NT2/D1) cells lines
Relations
BioProject PRJNA122657

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16858_RAW.tar 75.6 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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