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Series GSE168894 Query DataSets for GSE168894
Status Public on Aug 22, 2021
Title Role of Su(Hw) in DNA amplification in Drosophila ovaries
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Summary Suppressor of Hairy wing (Su(Hw)) is an insulator protein that participates in regulating chromatin architecture and gene repression in Drosophila. In previous studies we have shown that Su(Hw) is also required for pre-replication complex (pre-RC) recruitment on Su(Hw)-bound sites (SBSs) in Drosophila S2 cells and pupa. Here, we describe the effect of Su(Hw) on developmentally regulated amplification of 66D and 7F Drosophila amplicons in follicle cells (DAFCs), widely used as models in replication studies. We show Su(Hw) binding co-localizes with all known DAFCs in Drosophila ovaries, whereas disruption of Su(Hw) binding to 66D and 7F DAFCs causes a two-fold decrease in the amplification of these loci. The complete loss of Su(Hw) binding to chromatin violates pre-RC recruitment to all amplification regulatory regions of 66D and 7F loci at early oogenesis (prior to DAFCs amplification). These changes coincide with a considerable Su(Hw)-dependent condensation of chromatin at 66D and 7F loci. Although we observed the Brm, ISWI, Mi-2, and CHD1 chromatin remodelers at SBSs genome wide, their remodeler activity does not appear to be responsible for chromatin decondensation at the 66D and 7F amplification regulatory regions. We have discovered that, in addition to the CBP/Nejire and Chameau histone acetyltransferases, the Gcn5 acetyltransferase binds to 66D and 7F DAFCs at SBSs and is dependent on Su(Hw). We propose that the main function of Su(Hw) in developmental amplification of 66D and 7F DAFCs is to establish a chromatin structure that is permissive to pre-RC recruitment.
 
Overall design Ovaries were dissected from two developmental groups of females: 15 hours after eclosion (include egg chamber stages 1-8) and 32-34 hours after eclosion (include egg chamber 1-13).
 
Contributor(s) Vorobyeva NE, Mazina MY
Citation(s) 34417521
Submission date Mar 15, 2021
Last update date Aug 24, 2021
Contact name Nadezhda E Vorobyeva
E-mail(s) nvorobyova@gmail.com
Phone +79262790219
Organization name Institute of Gene Biology RAS
Street address Vavilova 34/5
City Moscow
ZIP/Postal code 119334
Country Russia
 
Platforms (1)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (39)
GSM5171996 Total_DNA_stock_Oregon_ovary_1-13_chambers_rep1
GSM5171997 Total_DNA_stock_Oregon_ovary_1-13_chambers_rep2
GSM5171998 Total_DNA_stock_SuHw_vf__ovary_1-13_chambers_rep1
Relations
BioProject PRJNA714523
SRA SRP310636

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Supplementary file Size Download File type/resource
GSE168894_RAW.tar 2.3 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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