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| Status |
Public on Nov 01, 2021 |
| Title |
Replicative history marks transcriptional and functional disparity in the memory T cell pool |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mice received DivisionRecorder+ or unmodified OT-I T cells and were challenged with LM-OVA. During memory phase we FACS sorted memory CD8+ OT-I T cells (and sub-sorted RFP+ and RFP- cells in the case of DivisionRecorder+ cells) from spleen material and performed single cell RNA sequencing.
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| Overall design |
Spleens were collected from LM-OVA infected recipient mice, DivisionRecorder+ or unmodified OT-I T cells were isolated by FACS, after which cells were processed for scRNA-seq (10X Chromium 3' kit), and sequenced on Illumina NextSeq
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| Contributor(s) |
Bresser K, Kok L, Schumacher T |
| Citation(s) |
35393592 |
| Submission date |
Mar 18, 2021 |
| Last update date |
May 02, 2022 |
| Contact name |
Kaspar Bresser |
| E-mail(s) |
k.bresser@sanquin.nl
|
| Organization name |
Sanquin
|
| Department |
Hematopoeisis
|
| Street address |
Plesmanlaan 125
|
| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
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| Samples (2) |
| GSM5177035 |
DivisionRecorder+ memory OT-I T cells |
| GSM5600726 |
DivisionRecorder+ and unmodified memory OT-I T cells |
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| Relations |
| BioProject |
PRJNA715447 |
| SRA |
SRP311211 |
