|
| Status |
Public on May 24, 2021 |
| Title |
Effect of fasting and ketogenic diet on hippocampal astrocytes in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the transcriptional effect of fasting and feeding a ketogenic diet on mouse CNS astrocytes, we performed translating ribosomal affinity purification (TRAP) of mRNAs immunoprecipitated from hippocampus. TRAP mice express a ribosomal epitope tag upon Cre-induced recombination that can be immunoprecipitated following activation. We measured the abundance of actively translating mRNAs from a ribosomal pull-down that came from adult astrocyte (Aldh1l1-Cre)-specific TRAP mice that were subjected to one of three dietary conditions: four weeks of normal chow diet, four weeks of ketogenic diet (high-fat, low-carbohydrate)43, or an 18-hour fast. Immediately following the respective diets, forebrain and hippocampus was harvested from all groups, ribosomes were immunoprecipitated, and actively translating mRNAs in the ribosomes were purified.
|
| |
|
| Overall design |
11 total samples were analyzed. 4 Fed, 3 Fasted, and 4 Ketogenic diet fed mice.
|
| |
|
| Contributor(s) |
Wolfgang MJ, Ellis JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 25, 2021 |
| Last update date |
May 24, 2021 |
| Contact name |
Michael J. Wolfgang |
| E-mail(s) |
mwolfga1@jhmi.edu
|
| Organization name |
The Johns Hopkins University School of Medicine
|
| Department |
Institute for Basic Biomedical Sciences
|
| Lab |
Rangos Bldg 475
|
| Street address |
855 N Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (11)
|
| GSM5211164 |
Hippocampal Astrocytes Fed Biological Sample 1 |
| GSM5211165 |
Hippocampal Astrocytes Fed Biological Sample 2 |
| GSM5211166 |
Hippocampal Astrocytes Fed Biological Sample 3 |
| GSM5211167 |
Hippocampal Astrocytes Fed Biological Sample 4 |
| GSM5211168 |
Hippocampal Astrocytes fasted Biological Sample 1 |
| GSM5211169 |
Hippocampal Astrocytes fasted Biological Sample 2 |
| GSM5211170 |
Hippocampal Astrocytes fasted Biological Sample 3 |
| GSM5211171 |
Hippocampal Astrocytes Ketogenic Biological Sample 1 |
| GSM5211172 |
Hippocampal Astrocytes Ketogenic Biological Sample 2 |
| GSM5211173 |
Hippocampal Astrocytes Ketogenic Biological Sample 3 |
| GSM5211174 |
Hippocampal Astrocytes Ketogenic Biological Sample 4 |
|
| Relations |
| BioProject |
PRJNA717239 |
Less...
More...