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| Status |
Public on Dec 22, 2009 |
| Title |
Potential mechanisms of low salt diet induced cardiac diseases |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
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| Summary |
We hypothesized that a LS intake leads to a cascade of events involving increases in plasma AII, the AT1 receptor, NADPH oxidase and O2˙ production and ultimately to reduced NO bioactivity. This reduced NO bioactivity results in altered control of the coronary circulation and cardiac metabolism. This study was undertaken to determine the global changes in cardiac gene expression in dogs fed LS diet (0.5% NaCl) for two weeks. Cardiac RNA was extracted from the control and LS fed groups (n=4). Statistical significance (P≤0.05; fold change of ±2) showed that there were 443 genes that changed, 110 decreasing and 333 increasing. All of the differentially expressed genes were grouped into functional categories based on their Gene Ontology.
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| Overall design |
Adult male mongrel dogs (n=10, BW;25-28kg) were put on LS which contains 0.5% sodium chloride for two weeks. RNA was isolated using Trizol reagent. RNA from each sample was used to generate a high fidelity cDNA using the labeling protocols for sample preparation recommended by Affymetrix. Data and statistical analysis were performed in Genetraffic using a t-test and with variance stabilization. Differences were considered statistically significant at a nominal significance of p≤0.05 and at least ±2 fold change in expression between control (n=3), and LS dogs (n=4).
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| Contributor(s) |
Suematsu N, Ojaimi c, Wang Z, Xu X, Zhang S, Recchia FA, Kaminski P, Sun D, Wolin MS, Kaley G, Hintze TH |
| Citation(s) |
20007914 |
| Submission date |
Jul 16, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Caroline Ojaimi |
| E-mail(s) |
CAROLINE_OJAIMI@NYMC.EDU
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| Phone |
914-594 4227
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| Organization name |
New York Medical College
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| Street address |
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| City |
Valhalla |
| State/province |
NY |
| ZIP/Postal code |
10595 |
| Country |
USA |
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| Platforms (1) |
| GPL3738 |
[Canine_2] Affymetrix Canine Genome 2.0 Array |
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| Samples (7)
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| GSM429071 |
Low Salt Diet project_control dog heart sample DC1 |
| GSM429072 |
Low Salt Diet project_control dog heart sample DC3 |
| GSM429073 |
Low Salt Diet project_control dog heart sample DC4 |
| GSM429074 |
Low Salt Diet project_LSD dog heart sample LSD1 |
| GSM429075 |
Low Salt Diet project_LSD dog heart sample LSD2 |
| GSM429076 |
Low Salt Diet project_LSD dog heart sample LSD3 |
| GSM429077 |
Low Salt Diet project_LSD dog heart sample LSD4 |
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| Relations |
| BioProject |
PRJNA119745 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17149_RAW.tar |
23.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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