|
| Status |
Public on Apr 07, 2021 |
| Title |
Direct Immunodetection of Global A-to-I RNA Editing Activity with a Chemiluminescent Bioassay |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We report the development of an ELISA-based chemiluminescent assay to measure global levels in A-to-I editing. We directly compare this performance to RNA-seq analysis using the Alu Editing Index. These attached datasets represent sequenced mRNA isolated from HEK293T cells transfected with increasing amounts of ADAR1 to induce a global increase in A-to-I RNA editing activity.
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| |
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| Overall design |
Measurement of global A-to-I editing activity in cellular mRNA.
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| |
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| Contributor(s) |
Knutson SD, Arthur RA, Johnston HR, Heemstra JM |
| Citation(s) |
33979483 |
| Submission date |
Apr 06, 2021 |
| Last update date |
Jul 07, 2021 |
| Contact name |
Steven Douglas Knutson |
| E-mail(s) |
steven.douglas.knutson@emory.edu
|
| Phone |
8479779677
|
| Organization name |
Emory University
|
| Department |
Chemistry
|
| Lab |
Heemstra
|
| Street address |
1515 Dickey Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA720037 |
| SRA |
SRP313642 |
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