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| Status |
Public on Apr 11, 2021 |
| Title |
RNA-sequencing and function analysis of adult gonad tissue identify potential key transcription factors in Macrobrachium resenbergii with sex determination |
| Organism |
Macrobrachium rosenbergii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
M. rosenbergii is a classical aquatic organism with sex-reversed phenomenon by gene-expressed regulation. Reversal of gonad development in M. rosenbergii received great attention but the role of transcription factors for gonadal maintenance and sex determination in adult shrimps remain unclearly. In this study, we sequencing the transcriptomes for the adult testis, ovary and androgenic gland by third-generation sequencing plus second-generation sequencing, and 21,345 were identified. In total, 9,199 differentially expressed genes (DEGs) were identified in pairwise comparison where 2,990 DEGs were upregulated expression in testis than other tissues, and 5,775 DEGs were upregulated expression in ovary, and 2,244 DEGs were upregulated expression in androgenic. There are 272 differentially expressed transcription factors (DETs) in total. The upregulated DETs in one of the testis, ovary and androgenic glands compared to the others were 107, 152 and 13 respectively. The GO and KEGG of DETs analysis suggested that MAPK signaling pathway and transcriptional misregulation pathway have key function in testis, the thyroid hormone signaling pathway and neuroactive ligand-receptor interaction play a important role in ovary. We determined the existence of networks containing significant DETs related to sex determination in the adult goands of M. rosenbergii. The key DETs are Piwi (only expression in testis and ovary) and Argonaute 2 (only expression in ovary) might be involved with the regulation of ovary development.
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| Overall design |
Three gonad mRNA profiles of adult male and female M.rosenbergii
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| Contributor(s) |
Ren J, Lou B, Niu B |
| Citation missing |
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| Submission date |
Apr 10, 2021 |
| Last update date |
Apr 11, 2021 |
| Contact name |
Jindong Ren |
| E-mail(s) |
renjindong@126.com
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| Organization name |
Zhejiang Academy of Agricultural Sciences
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| Street address |
No.198, Shiqiao Road
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| City |
Hangzhou |
| ZIP/Postal code |
310021 |
| Country |
China |
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| Platforms (1) |
| GPL29995 |
Illumina HiSeq 4000 (Macrobrachium rosenbergii) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA721068 |
| SRA |
SRP314298 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE171823_ExpressionProfileData.txt.gz |
557.4 Kb |
(ftp)(http) |
TXT |
| GSE171823_all_assembled_transcripts.fasta.gz |
9.9 Mb |
(ftp)(http) |
FASTA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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