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| Status |
Public on Sep 02, 2021 |
| Title |
Replication timing analysis in polyploid cells reveals Rif1 uses multiple mechanisms to promote underreplication in Drosophila. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Regulation of DNA replication and copy number are necessary to promote genome stability and maintain cell and tissue function. DNA replication is regulated temporally in a process known as replication timing (RT). Rif1 is key regulator of RT and has a critical function in copy number control in polyploid cells. In a previous study (Munden et al., 2018), we demonstrated that Rif1 functions with SUUR to inhibit replication fork progression and promote underreplication of specific genomic regions. How Rif1-dependent control of RT factors into its ability to promote underreplication is unknown. By applying a computational approach to measure RT in Drosophila polyploid cells, we show that SUUR and Rif1 have differential roles in controlling underreplication and RT. Our findings reveal that Rif1 functions both upstream and downstream of SUUR to promote underreplication. Our work provides new mechanistic insight into the process of underreplication and its links to RT.
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| Overall design |
Replication timing profiles prepared in salivary gland from female 3rd instar larvae prior to wandering and in fat body from female larvae 96 hours after egg laying (AEL).
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| Contributor(s) |
Das S, Caballero M, Kolesnikova T, Zhimulev I, Koren A, Nordman JT |
| Citation(s) |
34740250 |
| Submission date |
Apr 19, 2021 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Jared Nordman |
| E-mail(s) |
jared.nordman@vanderbilt.edu
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| Phone |
6158758365
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| Organization name |
Vanderbilt University
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| Department |
Biological Sciences
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| Lab |
Nordman Lab
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| Street address |
465 21st Ave S
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA723046 |
| SRA |
SRP315414 |
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