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| Status |
Public on Oct 12, 2021 |
| Title |
Comparative gene expression in adult SVZ-neurospheres differentiated under standard conditions and upon manipulations of Pax6 (OE) and/or Pbx1 (KD/OE) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarrays to detail the global programme of gene expression underlying neuronal differentiation of SVZ-neurospheres when manipulating Pax6 expression in the presence or absence of Pbx1
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| Overall design |
Freshly isolated stem- and progenitor cells from the SVZ of adult mice were subjected to four treatment regimes: i) infection with pCLIG, GFP-expressing retroviral vector viruses (vector ctrl), ii) infection with pCLIG expressing Pbx1 (Pbx1 OE), iii) transfection with non-targeting siRNAs followed by infection with pCLIG expressing Pax6 (Pax6 OE) and iv) transfection with siRNAs targeting Pbx1 followed by infection with pCLIG expressing Pax6 (Pax6 OE / Pbx1 KD). 48 hours later, the cells were differentiated for 10 hours on laminin-coated dishes by removal of EGF and FGF2 from the culture medium.
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| Contributor(s) |
Hau A, Schulte D |
| Citation(s) |
34697387 |
| Submission date |
Apr 20, 2021 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Ann-Christin Hau |
| E-mail(s) |
ann-christin.hau@lns.etat.lu
|
| Organization name |
Laboratoire National de sante
|
| Department |
National Center of Pathology
|
| Street address |
1, Rue Louis Rech
|
| City |
Luxembourg |
| ZIP/Postal code |
3555 |
| Country |
Luxembourg |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA723324 |
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