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| Status |
Public on Apr 22, 2021 |
| Title |
Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model for Multiple Sclerosis are distinct.
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| |
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| Overall design |
Comparison of DNA accessibility by Omni-ATAC-seq in sorted effector i- and m-T cells (mD2RED CD4+ CD44high) and naive i- and m-T cells (mD2RED CD4+ CD44int) from spleen.
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| |
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| Contributor(s) |
Korn T, Hiltensperger M, Lepennentier G, Moreno HD |
| Citation(s) |
34099917 |
| Submission date |
Apr 21, 2021 |
| Last update date |
Jun 20, 2021 |
| Contact name |
Thomas Korn |
| E-mail(s) |
thomas.korn@me.com
|
| Phone |
00498941405617
|
| Organization name |
Technical University of Munich
|
| Department |
Neurology
|
| Lab |
Korn lab
|
| Street address |
Ismaninger Str. 22
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA723595 |
| SRA |
SRP315764 |
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