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| Status |
Public on Jul 23, 2010 |
| Title |
The effect of IFNα on human CD8 T cells_with other concomitant signals |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
IFN alpha mediated gene expression pattern. The effect of IFN alpha on human CD8 T cells responding to antigen (signal 1) and costimulatory signals (signal 2) provided by beads coated with anti-CD3 and anti-CD28 mAbs.
This analysis examined the effects of IFN alpha on human CD8 T cells responding to antigen (signal 1) and costimulatory signals (signal 2) provided by beads coated with anti-CD3 and anti-CD28 mAbs. Magnetically sorted untouched CD8+CD45R0- T cells from three different donors were unstimulated or stimulated with IFNa2b or with anti-CD3/CD28 Beads alone or along with IFNa2b or IFNa5 for 48 hours. Individual mRNA samples were analyzed using HG-U133A 2.0 array gene chips.
Keywords: Gene expression analysis after different stimulation
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| Overall design |
Magnetically sorted untouched CD8+CD45R0-T cells from three different donors (named A, B and C) unstimulated or stimulated with IFN alpha 2b or with anti-CD3/CD28 beads alone or along with IFN alpha 2b or IFN alpha 5 for 48 hours.
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| Contributor(s) |
Hervás-Stubbs S, Riezu JI, González I, Mancheño U, Dubrot J, Azpilicueta A, Gabari I, Palazón A, Esquiroz P, Ruiz J, Prieto J, Le Bon A, Corrales F, Larrea E, Melero I |
| Citation(s) |
21108462 |
| Submission date |
Jul 24, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Iranzu Gonzalez |
| URL |
http://www.cima.es
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| Organization name |
Center for applied medical research
|
| Department |
Hepathology and Gene Therapy
|
| Lab |
3.03. Clinic immunology
|
| Street address |
Avda. Pio XII nº 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (15)
|
| GSM432933 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor A unstimulated |
| GSM432934 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor A stimulated with IFNα2 for 48 hours |
| GSM432935 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor A stimulated with anti CD3/CD8 beads for 48 hours |
| GSM432936 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor A stimulated with anti CD3/CD8 beads+IFNα2 for 48 hours |
| GSM432937 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor A stimulated with anti CD3/CD8 beads+IFNα5 for 48 hours |
| GSM432938 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor B unstimulated |
| GSM432939 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor B stimulated with IFNα2 for 48 hours |
| GSM432940 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor B stimulated with anti CD3/CD8 beads for 48 hours |
| GSM432941 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor B stimulated with anti CD3/CD8 beads+IFNα2 for 48 hours |
| GSM432942 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor B stimulated with anti CD3/CD8 beads+IFNα5 for 48 hours |
| GSM432943 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor C unstimulated |
| GSM432944 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor C stimulated with IFNα2 for 48 hours |
| GSM432945 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor C stimulated with anti CD3/CD8 beads for 48 hours |
| GSM432946 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor C stimulated with anti CD3/CD8 beads+IFNα2 for 48 hours |
| GSM432947 |
Magnetically sorted untouched CD8+CD45R0-T cells from donor C stimulated with anti CD3/CD8 beads+IFNα5 for 48 hours |
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| This SubSeries is part of SuperSeries: |
| GSE17302 |
IFNα-mediated gene expression patterns |
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| Relations |
| BioProject |
PRJNA123599 |
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