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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 08, 2012 |
| Title |
mRNA expression upon reconstitution of miR-130a, miR-203 and miR-205 in prostate cancer cell line LNCaP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Micro RNAs (miRNAs) miR-130a, miR-203 and miR-205 are jointly downregulated in prostate cancer and act as repressors of AR-signaling. MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. Reconstitution of these lost miRNAs in the LNCaP PCa cell line cause morphology changes, growth arrest, and apoptosis, increasing when the miRNAs were co-expressed. Bioinformatic target prediction, mRNA expression and protein expression analysis upon overexpression of these miRNAs congruently identified targets known to be overexpressed in PCa and to be involved in AR trans-activation. This series profiles loss in mRNA expression in LNCaP cells transfected with one of the three miRNAs miR-130a, miR-203 and miR-205 compared to LNCaP cells transfected with a scramble miRNA.
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| Overall design |
We analyzed three arrays each for miR-130a, miR-203, miR-205, and a scramble miRNA.
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| Contributor(s) |
Boll K, Reiche K, Mörbt N, Kretzschmar AK, Schutt K, Tomm J, Verhaegh G, Schalken J, von Bergen M, Horn F, Hackermüller J |
| Citation(s) |
22391564 |
| Submission date |
Jul 24, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Kristin Reiche |
| E-mail(s) |
kristin.reiche@izi.fraunhofer.de
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| Organization name |
Fraunhofer Institute for Cell Therapy and Immunology
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| Department |
Diagnostics
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| Street address |
Perlickstr. 1
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| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| GSM433191 |
miR-130a overexpression in LNCaP, biological replicate 1 |
| GSM433192 |
miR-130a overexpression in LNCaP, biological replicate 2 |
| GSM433193 |
miR-130a overexpression in LNCaP, biological replicate 3 |
| GSM433194 |
miR-203 overexpression in LNCaP, biological replicate 1 |
| GSM433195 |
miR-203 overexpression in LNCaP, biological replicate 2 |
| GSM433196 |
miR-203 overexpression in LNCaP, biological replicate 3 |
| GSM433197 |
miR-205 overexpression in LNCaP, biological replicate 1 |
| GSM433198 |
miR-205 overexpression in LNCaP, biological replicate 2 |
| GSM433199 |
miR-205 overexpression in LNCaP, biological replicate 3 |
| GSM433200 |
miRNA scramble expression in LNCaP, biological replicate 1 |
| GSM433201 |
miRNA scramble expression in LNCaP, biological replicate 2 |
| GSM433202 |
miRNA scramble expression in LNCaP, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE17362 |
miRNA expression, mRNA expression upon miRNA reconstitution, and direct mRNA target identification in prostate cancer cell lines |
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| Relations |
| BioProject |
PRJNA123603 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17315_RAW.tar |
18.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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