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| Status |
Public on Sep 27, 2021 |
| Title |
Blood transcriptomes of anti-SARS-CoV2 antibody positive healthy individuals with prior asymptomatic versus clinical infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite tremendous efforts by the international research community to understand the pathophysiology of SARS-CoV-2 infection, the reasons behind the clinical variability, ranging from asymptomatic infection to lethal disease, are still unclear. Existing inter-individual variations of the immune responses, due to environmental exposures and genetic factors, may be critical to the development or not of symptomatic disease after infection with SARS-CoV-2, and transcriptomic differences marking such responses may be observed even later, after convalescence. Herein, we performed genome-wide transcriptional whole-blood profiling to test the hypothesis that immune response-related gene signatures may differ between healthy individuals with prior entirely asymptomatic versus clinical SARS-CoV-2 infection, all of which developed an equally robust antibody response. Among 12.789 protein-coding genes analyzed, there were only six and nine genes with significantly decreased or increased expression, respectively, in those with prior asymptomatic infection (n=17, mean age 34 years) relatively to those with clinical infection (n=15, mean age 37 years). All six genes with decreased expression (IFIT3, IFI44L, RSAD2, FOLR3, PI3, ALOX15), are involved in innate immune response while the first two are interferon-induced proteins. Among genes with increased expression six are involved in immune response (GZMH, CLEC1B, CLEC12A), viral mRNA translation (GCAT), energy metabolism (CACNA2D2) and oxidative stress response (ENC1). Notably, 8/15 differentially expressed genes are regulated by interferons. Our results suggest that an intrinsically weaker expression of some innate immunityrelated genes may be associated with an asymptomatic disease course in SARS-CoV-2 infection. Whether a certain gene signature predicts, or not, those who will develop a more efficient immune response upon exposure to SARS-CoV-2, with implications for prioritization for vaccination, warrant further study.
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| Overall design |
32 human whole blood samples of anti-SARS-CoV2 antibody positive healthy individuals with prior asymptomatic (n=17) versus clinical infection (n=15)
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| Contributor(s) |
Sfikakis PP, Verrou K, Tsitsilonis O, Paraskevis D, Kastritis E, Lianidou E, Moutsatsou P, Terpos E, Trougakos I, Chini V, Manoloukos M, Moulos P, Pavlopoulos GA, Kollias G, Ampatziadis-Michailidis G, Hatzis P, Dimopoulos MA |
| Citation(s) |
34675930 |
| Submission date |
Apr 26, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Maria G. Tektonidou |
| E-mail(s) |
mtektonidou@gmail.com
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| Organization name |
National and Kapodistrian University of Athens
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| Street address |
Mikras Asias 75
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| City |
Athens |
| State/province |
Please choose a State |
| ZIP/Postal code |
11527 |
| Country |
Greece |
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| Platforms (1) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA725183 |
| SRA |
SRP316381 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE173317_RAW.tar |
731.8 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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